With years of experience and high-end technologies in antibody engineering, Creative Biolabs has successfully developed a series of innovative and diversified antibody platforms to provide fast and convenient non-IgG antibodies (IgM, IgE or IgA) humanization services for our worldwide customers.
Antibody Humanization
Antibodies have become an effective tool in various human diseases treatment and diagnosis, and they play an essential role in the remarkable progress of antibodies as therapeutic molecules. Antibody humanization is necessary and of great significance when these therapeutic monoclonal antibodies are produced from non-human systems. It can decrease the immunogenicity, improve its pharmacokinetics in vivo, and maintain high affinity and specificity of monoclonal antibodies (mAbs) by antibody humanization engineering.
Heretofore, different methods and techniques have been developed for the humanization of non-human antibodies to improve their biological properties. Currently, antibody humanization can be conducted by several engineering techniques, such as complementarity determining the region (CDR) grafting, chain shuffling, resurfacing and antibody library selections.
With the development of research on bioactive molecules, not only IgG mAbs but also non-IgG antibodies play an increasingly important role in the diagnosis and treatment of diseases. It is necessary to develop humanization engineering techniques for non-IgG therapeutic antibodies. At Creative Biolabs, high-quality non-IgG antibodies (mainly IgA, IgM, and IgE) are generated by our Expression System Platform through DNA recombinant technologies. For non-IgG therapeutic antibodies that are obtained from non-human expression systems, we optimize them through humanization engineering technique to enhance their performance.
Antibody Humanization Services at Creative Biolabs
Humanization of non-IgG antibodies usually conducted via CDR grafting at the DNA level, meaning that inserting appropriate or designed CDR coding segments into a human antibody framework. Once your non-IgG antibody is obtained, scientists at Creative Biolabs can start with DNA sequence determination of your non-IgG antibodies CDRs and provide professional humanization services based on our advanced technology platform. Our humanized non-IgG antibodies services mainly include, but are not limited to:
- Antibody humanization design
- Vector synthesis and cloning
- Transient expression
- Stable expression of cell lines
Fig.1 Schematic overview of antibody humanization from murine antibodies (red) to fully human antibodies (blue). (Santos, 2018)
Features of Our Services
➢ High affinity and specificity
➢ Wide range of antibody species
➢ Immunogenicity evaluation in primates
➢ Abundant humanization service experience
➢ Constant quality
As a professional service supplier of therapeutic or diagnostic antibody development, Creative Biolabs has extensive expertise and research experience to offer antibody humanization services. Built on decades of combined experience in the antibody development industry, our patented platform enables us to offer a robust, reliable and accurate modernized version of the non-IgG antibodies humanization services. If you are interested in our services, please don't hesitate to contact us for more information.
Reference
- Santos, M.L.; et al. Advances and challenges in therapeutic monoclonal antibodies drug development. Brazilian Journal of Pharmaceutical Sciences. 2018, 54(SPE).
KINDLY NOTE
!! For Research Use Only. Our products and services are NOT intended for diagnostic or therapeutic applications.
