Antibody Isotype Switching Service

As a senior expert in antibody development, Creative Biolabs owns lots of scientists who are proficient in non-IgG therapeutic antibody production, engineering, and application. Our professional scientists are pleased to provide the custom non-IgG antibody isotype switching service against diverse disease biomarkers to diagnose and treat related diseases efficiently. We believe our first-class services will fit your needs in your most critical research requirements.

For many indication therapies, choosing the appropriate antibody isotype and format is as critical as having the right antigen specificity. Creative Biolabs can help you obtain a suitable isotype based on your parental antibody and intended application. Utilizing our advanced antibody engineering techniques and expertise, our scientists are able to switch antibody isotypes among these five isotypes (IgG, IgA, IgD, IgE, and IgM) of any species.

Our Service

Antibody isotype switching service is a specialized solution designed to assist researchers in converting an antibody from one immunoglobulin isotype (e.g., IgM) to another (e.g., IgG, IgA, or IgE). This service is crucial for optimizing antibody functionality, enhancing specificity, and studying the role of antibodies in immune responses.

Service Process

Highlights

• Precise Isotype Conversion
  Achieve accurate antibody isotype switching by targeting the immunoglobulin heavy chain constant region, ensuring seamless conversion to the desired isotype.
• Customizable Solutions for Specific Needs
  Tailor-made antibody isotype options, such as IgA, IgM, or IgE, to meet diverse research and application requirements.
• Enhanced Antibody Functionality
  Improve antibody properties like stability, effector functions, and compatibility with Fc-mediated mechanisms such as ADCC and CDC.
• High Purity and Yield
  Advanced purification techniques ensure high-quality, purified antibody products suitable for critical downstream applications.
• Broad Range of Applications
  Supports various fields, including vaccine development, immunotherapy research, and basic studies in immune mechanisms.
• Optimized Expression Systems
  Utilize HEK 293 or CHO cells with optimized culture conditions for efficient antibody expression and correct folding.
• Time and Cost Efficiency
  Streamlined processes reduce research timelines and deliver ready-to-use antibodies, saving time and resources for researchers.
• Integrated Reporting and Support
  Detailed experimental reports with antibody sequence, validation data, and performance metrics, providing full transparency and support.

Background

In the human body, immunoglobulin class switching takes place rapidly after the activation of mature naive B cells, leading to a switch from the expression of IgM and IgD to the expression of IgG, IgE, or IgA. The first class of immunoglobulin produced in an immune response is IgM. With the immune response progressing, the immunoglobulins produced by B cells change from predominantly IgM to IgG, IgE, or IgA classes depending upon the type of stimulation. In fact, only the constant region of the antibody's heavy chain is altered while the variable region of the heavy chain remains invariable during this process, which results in changes in antibody binding to different effector molecules rather than changes in antigen specificity. This switch can promote the ability of antibodies to remove the pathogen by inducing the immune response that they are expert in.

In vitro, class switching occurs by the deletional recombination between two switch regions, each of which is associated with an H chain constant region gene. During the class-switch recombination (CSR), the constant region of the antibody heavy chain is changed, but the variable region of the heavy chain remains the same. Similarly, this recombinant switching does not change antigen specificity since the variable region does not alter. CSR is initiated by activation-induced cytidine deaminase, which converts cytosines in switching regions to uracils. The uracils are subsequently removed by two DNA-repair pathways, resulting in mutations, single-strand DNA breaks, and the double-strand breaks required for CSR.

Fig. 1 Outline of loop extrusion and class switch recombination center (CSRC) model.¹

FAQs

Resource

Switching an antibody to a suitable isotype can help it gain better performance in effector functions and disease therapies. As a senior company in antibody research and service providing, Creative Biolabs has bent itself into non-IgG antibody exploration for several years to improve the abilities of our comprehensive antibody services. We can customize our isotype switching services according to your needs and purpose using our extensive antibody engineering technologies and expertise. What you need to do is just directly contact us.

Quotation and Ordering

Our customer service representatives are available 24/7/365. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email, or by fax.

Reference

1. Dauba, Audrey, and Ahmed Amine Khamlichi. "Long-range control of class switch recombination by transcriptional regulatory elements." Frontiers in Immunology 12 (2021): 738216. Distributed under the Open Access license CC BY 4.0, without modification.

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