As a long-term expert in antibody development, Creative Biolabs has gathered lots of scientists who are proficient in non-IgG antibodies development. Our professional scientists are pleased to provide the custom non-IgG antibody products and comprehensive services for different purposes and applications. Armed with years of experience and advanced high-throughput technologies, we offer one-stop non-IgG development services extended from antibody production & purification to characterization & modification.
Antibody Characterization
The production of antibodies with stable properties, predictable, and most importantly, the desired performance is a rather long process. Antibody characterization is quite an important process for further research and biopharmaceutical development due to the heterogeneities caused by modifications during different stages of production and purification. In order to figure out and elucidate how your antibody performs and functions in your projects, antibody characterization is fundamental and indispensable. Strategies or solutions for antibody characterization depend on many factors, such as quantities of available antibodies, properties, intended application, and so forth.
Several techniques are available at Creative Biolabs to evaluate the quality, quantity, and function of the therapeutic non-IgG antibodies produced, such as enzyme-linked immunosorbent assay (ELISA), flow cytometry, immunoblotting, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, our innovative protein analytical methods can provide you with important and detailed information about IgA, IgE, and IgM therapeutic antibodies and help you screen throughout the development phase.
Fig.1 Immunoglobulin isotypes and their sites of glycosylation. (Maverakis, 2015)
Technology for Non-IgG Antibody Characterization at Creative Biolabs
After gaining high-quality non-IgG antibodies by our production and purification, Creative Biolabs provides a series of strategic approaches with detailed characterization of your antibody, including but not limited to:
- Antibody titration and quantization by ELISA
- High-performance liquid chromatography (HPLC) analysis
- Intact mass determination by mass spectrometry of heavy and light chains
- N- and C-terminal sequencing of the heavy and light chain by Edman degradation and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)
- Western blot (WB) assay
- SDS-PAGE assay
- Epitope binning
- Affinity ranking
- EC50 and IC50
- Cross-reactivity
- Cell-based screening
Creative Biolabs has excellent technical knowledge and long-standing experience in the detailed characterization of different formats of non-IgG antibodies. We provide both individual analyses and a comprehensive characterization package customized to suit your requirements. Additionally, related non-IgG antibody services, such as engineering, production and purification, and PK/PD evaluation, are also within the field we are skilled in.
If you are interested in our antibody characterization services, please feel free to contact us for more information.
Reference
- Maverakis, E.; et al. Glycans in the immune system and The Altered Glycan Theory of Autoimmunity: a critical review. Journal of autoimmunity. 2015, 57: 1-13.
KINDLY NOTE
!! For Research Use Only. Our products and services are NOT intended for diagnostic or therapeutic applications.
