As a pioneer company in the field of non-IgG therapeutic antibodies engineering, Creative Biolabs has successfully established an advanced technology platform for the production of numerous types of recombinant antibody. Here, we are happy to introduce our high-quality chimeric IgM antibody engineering services for global customers.
Introduction of IgM Antibody
IgM is the first class of antibody that is synthesized by and appears on the surface of a developing B cell, although many B cells eventually switch to other classes. It is also the major class that is secreted into the blood in the early stages of a primary antibody response on initial exposure to an antigen. As the first line of defense to an organism, IgM usually exists as polymers that comprise five or six 4-chain units in its secreted form, which results in a total of 10 targets-binding sites and thus higher valency than other isotypes. In addition, this structure allows its binding to antigens with high avidity. Studies have shown that IgM is involved in various physiological processes, such as regulating B cell development, facilitating the clearance of apoptotic cells, modulating inflammatory responses and mediating the elimination of cancer cells.
Fig.1 Natural IgM is produced by B1 cells and marginal zone cells, and adaptive IgM is synthesized by B2 cells. (Díaz-Zaragoza, 2015)
Chimeric IgM Antibody Engineering
Recently, increasing investigations have highlighted the essential and potential roles of IgM in the tissue homeostasis maintenance, inflammation inhibition, misfolded or altered proteins removal, and so forth. What’s more, in the field of tumor biology and therapy, IgMs can more effectively target some cancer cell markers with higher avidity compared to those IgG class antibodies. However, the clinical use of murine monoclonal antibodies (mAbs) is hampered by their short half-life and potential to elicit an anti-(mouse Ig) response, which may interfere with therapy. Fortunately, advances in antibody engineering, such as chimeric modification and humanization, have largely improved the performance of these murine mAbs and made it possible to overcome these limitations.
During the past years, chimeric IgM has been developed, which was conducted by incorporation of a given murine mAb (both the heavy- and light- chain variable regions) into an expression plasmid containing the constant (Cμ) region of human IgM. Upon successful transfection, the cells containing constructed plasmid expresses a human-murine hybrid (chimeric) IgM molecule, which reacts like human IgM while retaining the specificity of the "parent" murine mAb.
Chimeric IgM Antibody Engineering Services Provided by Creative Biolabs
Aided by our top technology and years of experience, we are able to offer a series of antibody engineering services with the best quality. Our chimeric antibody engineering service allows you to rapidly convert the species isotype of your antibody to validate its potential in various therapeutic or diagnostic assays. The chimeric IgM antibody services we offer include but are not limited to:
- Plasmid construction
- Chimeric antibody production and purification
- Antibody characterization
- Chimeric non-IgG antibodies application
Creative Biolabs is committed to accelerating the project development of our worldwide customers. In addition to IgM, we also offer chimeric antibody engineering services for other antibody classes. If you are interested in the services we provide, please feel free to contact us for more information.
Reference
- Díaz-Zaragoza, M.; et al. Natural and adaptive IgM antibodies in the recognition of tumor-associated antigens of breast cancer. Oncology reports. 2015, 34(3): 1106-1114.
KINDLY NOTE
!! For Research Use Only. Our products and services are NOT intended for diagnostic or therapeutic applications.