With abundant experience in recombinant antibody development, Creative Biolabs is able to provide a full range of chimeric antibody engineering services for global customers. Our professional team is optimized to help you with high-quality and cost-effective services to make your project a success. Especially, we are capable of offering high-quality chimeric IgE antibody production service.
Introduction to IgE Antibody
IgE antibody is the least abundant circulating immunoglobulin isotype in mammal serum. IgE mediates various functions through binding to 2 Fcε receptors (FcεRs) mainly on the surface of mast cells and basophils, the high-affinity receptor FcεRI and the low-affinity receptor FcεRII (CD23). As an important member of humoral immunity, IgE triggers immune reactions for host defense to protect against parasitic worms (helminths) and eliminate environmental substances including toxins, venoms, irritants, and xenobiotics. Studies have shown that IgE antibodies play an essential role in inducing the allergic immune response (mainly type I hypersensitivity) when exposed to allergens, which results in mast cells and/or basophil degranulation, acute inflammation, and various allergic diseases. Furthermore, increasing evidence suggests that IgE may play a significant role in antitumor immunity. Especially, several chimeric IgE specific for certain cancer antigens recently have exhibited rather favorable anti-tumor effects.
Fig.1 Organization and conformational rearrangements of the IgE Fc. (Jabs, 2018)
Chimeric IgE Antibody Engineering
During the past years, though the IgE functions have been extensively investigated, the applications of IgE for clinical use are hampered as the expression level of nature IgE antibody is very low and it may elicit an unwanted immune response. In this case, IgE antibodies chimerized by replacing the antibody constant region with other species to reduce the antibody's immunogenicity while retaining the ability to specifically bind to the antigen have been widely used for antibody preparation. By this modification, chimeric IgE antibodies reduce anti-species immune responses when used in different animal models, particularly for human applications. For instance, mouse-human chimeric IgE antibody specific for the epithelial antigen MUC-1 (anti-MUC-1 IgE), mouse-human chimeric IgE against a prostate-specific antigen (anti-PSA IgE), anti-Ly-2 rat/human chimeric IgE, have been widely evaluated in IgE-based cancer therapies in vivo.
Chimeric IgE Antibody Engineering Services Provided by Creative Biolabs
With years of experience and advanced technology platforms in chimeric antibody production, Creative Biolabs not only provides human and mouse antibodies but also rabbit, rat, canine antibodies; not only IgG isotype but also non-IgG isotypes (mainly IgA, IgM, and IgE). Especially, we have accumulated hands-on experience in expressing and producing the chimeric form of the IgE antibodies using our cutting-edge transient transfection platform, which can offer great services to customers worldwide.
Creative Biolabs is committed to accelerating the development of our clients' chimeric antibody programs. Once you provide the antibody sequence or plasmid, we can tailor the project to meet your specific requirements. More importantly, we also offer related non-IgG antibodies services, such as target diseases, pre-made or customized antibody products, and relevant applications. For more detailed information, please feel free to contact us or directly send us an inquiry.
Reference
- Jabs, F.; et al. Trapping IgE in a closed conformation by mimicking CD23 binding prevents and disrupts FcεRI interaction. Nature communications. 2018, 9(1): 7.
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!! For Research Use Only. Our products and services are NOT intended for diagnostic or therapeutic applications.
