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This product is an unconjugated anti-POLR2A Monoclonal antibody (CTD 8A7) generated from the Mouse. This antibody can be used for WB, ELISA, FC, IF.
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specifications |
|
|---|---|
| Antibody Isotype | IgM |
| Clone | CTD 8A7 |
| Applications | WB; ELISA; FC; IF |
| Target | POLR2A |
| Host | Mouse |
| Clonality | Monoclonal |
| Antibody Type | Primary antibody |
| Species Reactivity | Human, Yeast |
| Immunogen | 10 repeats of synthetic peptide YSPTSPS using chemically sythesized phospho-Ser5 |
| Format | Liquid |
| Buffer | PBS |
| Storage | Store at 4° C for short term and at -20° C or lower for long term. Avoid freeze-thaw cycles. |
Target Information |
|
|---|---|
| Target Name | POLR2A |
| Alternative Names | RNA Polymerase II Subunit A; RNA Polymerase II Subunit B1; DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit; Polymerase (RNA) II (DNA Directed) Polypeptide A 220kDa; DNA-Directed RNA Polymerase III Largest Subunit; DNA-Directed RNA Polymerase II Subunit RPB1; DNA-Directed RNA Polymerase II Subunit A; POLR2; POLRA; RPB1; Polymerase (RNA) II Subunit A; HRPB220; NEDHIB; RpIILS; HsRPB1; RPBh1; RPOL2; RPO2 |
| Related Disease | Neurodevelopmental Disorder With Hypotonia And Variable Intellectual And Behavioral Abnormalities; Hypotonia |
| Gene ID | 430 |
| UniProt ID | P24928 |
| Target Overview | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression. It acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. |
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