Mouse Anti-NFkB p105 IgM Monoclonal Antibody (A2-7)

specifications
Antibody Isotype	IgM
Clone	A2-7
Applications	WB; IF; IHC
Target	NFkB p105
Host	Mouse
Clonality	Monoclonal
Antibody Type	Primary antibody
Species Reactivity	Human, Mouse
Immunogen	A synthetic peptide (KLH-coupled) corresponding to NFkB p105
Format	Liquid
Buffer	TBS with 1%BSA and 40%Glycerol, pH7.4
Storage	Store at 4° C for short term and at -20° C or lower for long term. Avoid freeze-thaw cycles.

specifications

Antibody Isotype

IgM

Clone

A2-7

Applications

WB; IF; IHC

Target

NFkB p105

Host

Mouse

Clonality

Monoclonal

Antibody Type

Primary antibody

Species Reactivity

Human, Mouse

Immunogen

A synthetic peptide (KLH-coupled) corresponding to NFkB p105

Format

Liquid

Buffer

TBS with 1%BSA and 40%Glycerol, pH7.4

Storage

Store at 4° C for short term and at -20° C or lower for long term. Avoid freeze-thaw cycles.

Target Information
Target Name	NFkB p105
Alternative Names	Nuclear Factor Kappa B Subunit 1; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear Factor NF-Kappa-B P105 Subunit; DNA-Binding Factor KBF1; NF-KappaB; NFKB-P50; NFkappaB; NF-KB1; EBP-1; KBF1; Nuclear Factor Kappa-B DNA Binding Subunit; Nuclear Factor NF-Kappa-B P50 Subunit; NF-Kappabeta; NF-Kappa-B1; CVID12; NF-KB; NFKB1; P105; P50
Related Disease	Immunodeficiency Common Variable 12; Common Variable Immunodeficiency
Gene ID	4790
UniProt ID	P19838
Target Overview	NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Target Information

Target Name

NFkB p105

Alternative Names

Nuclear Factor Kappa B Subunit 1; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear Factor NF-Kappa-B P105 Subunit; DNA-Binding Factor KBF1; NF-KappaB; NFKB-P50; NFkappaB; NF-KB1; EBP-1; KBF1; Nuclear Factor Kappa-B DNA Binding Subunit; Nuclear Factor NF-Kappa-B P50 Subunit; NF-Kappabeta; NF-Kappa-B1; CVID12; NF-KB; NFKB1; P105; P50

Related Disease

Immunodeficiency Common Variable 12; Common Variable Immunodeficiency

Gene ID

4790

UniProt ID

P19838

Target Overview

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

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