IgY Production and Purification Service

Our Service Highlights Background FAQs Resource Quotation and Ordering

IgY antibodies are immunoglobulins produced by birds and some amphibians. They are similar in structure to human IgG, but have unique biological properties. They are mainly found in egg yolks and serum, and can effectively identify and neutralize pathogens and fight infections. At the same time, due to their low allergenicity and good safety, they are increasingly used in research and product development in the fields of medicine, veterinary medicine, and food safety. Creative Biolabs provides professional IgY production and purification services, with a variety of methods to choose from, to help your non-igg antibody projects.

Our Service

At Creative Biolabs, we have developed a comprehensive IgY platform to address global needs in research, diagnostics, and industrial applications. Our services include:

IgY Production

Creative Biolabs offers a streamlined process for the production of high-quality IgY antibodies:

  • Antigen Preparation: We provide custom antigen design and synthesis to ensure optimal immune response in hens.
  • Hen Immunization: Using ethically approved protocols, we immunize hens to generate IgY antibodies specific to your targets.
  • Egg Collection: Post-immunization, IgY antibodies are harvested directly from the egg yolks, ensuring a non-invasive, cost-effective approach.

IgY Isolation

Our platform supports a variety of isolation techniques tailored to your specific requirements:

  • Organic Precipitation: Efficiently remove lipids using methods like polyethylene glycol (PEG) or dextran sulfate.
  • Organic Solvent Extraction: Separate IgY from yolks using solvents like chloroform under optimized conditions.
  • Natural Gum Method: Utilize carrageenan or xanthan gum for lipid removal with minimal protein loss.
  • Medium-Chain Fatty Acid Method: Use caprylic acid for effective IgY isolation.
  • Detergent-Based Separation: Employ surfactants like Triton X-114 for high recovery rates.
  • Water Dilution Method: A simple and economical method to extract IgY.
  • Supercritical Fluid Extraction: Advanced lipid removal using CO₂ under supercritical conditions.

IgY Purification

Our purification techniques ensure high-purity IgY for research and industrial needs:

  • Ultrafiltration: Concentrate and desalinate IgY with molecular weight cut-off membranes.
  • Inorganic Precipitation: Use ammonium or sodium sulfate for scalable IgY purification.
  • Organic Solvent Precipitation: Combine solvents like ethanol with PEG for enhanced purity.

IgY Advanced Purification

For applications demanding ultra-pure IgY, we employ:

  • Gel Filtration Chromatography: Achieve precise molecular size separation.
  • Ion Exchange Chromatography: Enhance purity based on charge properties.
  • Affinity Chromatography: Utilize specialized interactions such as thiophilic or metal ion affinity chromatography.

Highlights

  • Comprehensive IgY Production Workflow
    Creative Biolabs offers end-to-end solutions for IgY antibody production, from antigen preparation and hen immunization to ethical egg collection. Our streamlined process ensures cost-effective and high-quality antibody development.
  • Advanced IgY Isolation Techniques
    Choose from a variety of isolation methods, including organic precipitation, solvent extraction, and supercritical fluid extraction, tailored to meet your research or industrial requirements.
  • High-Purity IgY Antibodies
    Our purification platform employs cutting-edge technologies such as ultrafiltration, ion exchange chromatography, and affinity chromatography to deliver ultra-pure IgY antibodies suitable for diagnostics and research.
  • Ethical and Sustainable Approach
    IgY extraction eliminates the need for blood collection, minimizing animal distress. This humane and sustainable technology aligns with modern research ethics and industry standards.
  • Customizable Solutions for Non-IgG Antibodies
    With years of expertise in antibody development, Creative Biolabs provides fully customizable services for IgY and other non-IgG antibodies (covering IgA, IgE, and IgM antibodies), addressing diverse scientific challenges.
  • Expertise in Diagnostics and Research Applications
    Our IgY antibodies, known for their specificity and stability, are ideal for applications in pathogen detection, immune system research, and diagnostic assay development.

Background

IgY antibodies, the avian equivalent of mammalian IgG, exhibit structural and functional similarities but also key differences. While both molecules possess two Fab arms, IgY has four heavy-chain constant domains, compared to the three in IgG. The molecular weight of IgY is approximately 167 kDa, slightly heavier than IgG, as determined by mass spectrometry. Under SDS-PAGE analysis, IgY shows bands at ~70 kDa (heavy chain) and ~25 kDa (light chain). IgY antibodies are remarkably stable under diverse pH conditions and high temperatures, making them ideal for a range of research and diagnostic applications. The production, extraction, and application of IgY antibodies from egg yolks represent an innovative and humane approach to antibody development. Unlike traditional mammalian antibodies, IgY extraction does not involve blood collection, significantly reducing animal harm. Moreover, the phylogenetic distance between birds and mammals allows IgY to target conserved mammalian proteins or immune-evading antigens more effectively. IgY antibodies also lack Fc receptor binding and do not activate mammalian complement systems, minimizing background interference in diagnostic platforms.

Fig. 1 Comparison of the structures of avian IgY and mammalian IgG.Fig. 1 Structure of avian IgY versus mammalian IgG.1

FAQs

Q1: What are the advantages of IgY antibodies over traditional IgG?

A: IgY offers reduced non-specific interactions, no binding to mammalian Fc receptors, and no activation of mammalian complement systems. These features minimize interference in diagnostic assays and improve specificity.

Q2: Can IgY antibodies be purified to high purity levels?

A: Yes, IgY can be purified to high levels using techniques such as ultrafiltration, inorganic precipitation with ammonium sulfate, and chromatography methods like ion exchange and affinity chromatography.

Q3: Are IgY antibodies stable in extreme conditions?

A: IgY antibodies exhibit remarkable stability across a wide pH range and at elevated temperatures, making them suitable for diverse experimental and storage conditions.

Q4: What purification methods are best for large-scale IgY production?

A: For industrial-scale production, methods like PEG precipitation combined with ultrafiltration or ion exchange chromatography are cost-effective and scalable, ensuring high yield and purity.

Q5: Why should I choose Creative Biolabs for IgY services?

A: Creative Biolabs offers expertise in IgY production and purification, providing customizable solutions, advanced technology, and ethical practices. With years of experience in non-IgG antibodies, we deliver high-quality products tailored to your specific research needs.

Resource

Youtube A Brief Introduction to Non-IgG Antibody - Creative Biolabs spotify A Brief Introduction to Non-IgG Antibody - Creative Biolabs

If you are interested in our IgY production and purification services or have inquiries regarding non-IgG antibodies, please feel free to contact us for more information. Let Creative Biolabs be your trusted partner in antibody innovation.


Quotation and Ordering

Our customer service representatives are available 24/7/365. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email, or by fax.

Fill out and submit the online inquiry form.
A Technical Account Manager will respond within 24 hours to discuss the service details and finalize the quote.
Sign the order.
Your project services will commence immediately.
A Project Manager will stay in touch with you throughout the production process to provide updates on the project's progress.
Deliverables will be provided along with the relevant experimental data.

Reference

  1. Müller, Sandra, et al. "IgY antibodies in human nutrition for disease prevention." Nutrition Journal 14 (2015): 1-7. Distributed under the Open Access license CC BY 4.0, without modification.
KINDLY NOTE

For Research Use Only. Our products and services are NOT intended for diagnostic or therapeutic applications.

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